The ABI PRISM 3730 & 3100 Genetic Analyzers
are state of the art machines
for automated DNA capillary
electrophoresis sequencing &
genotyping. Any primer can be
used (provided by the customer),
and we will guarantee more than
800 bases of good quality
fluorescence-based DNA sequence.
As with all sequencing, the
quality of the sequence is
dependent on the quality of the
template DNA and primer as well
as careful quantitation of the
DNA concentration.
Samples will be accepted in the
form of highly purified DNA
template
Templates should be
provided at the following
concentrations:
|
Template |
Concentration |
|
Plasmid |
300 ng/ml |
|
Cosmid |
1
mg/ml |
|
BAC |
1
mg/ml |
|
Bacterial Genomic |
3
mg/ml |
|
Phage |
1
mg/ml |
|
Template |
Concentration |
|
PCR Fragment 100 - 200
bp |
3 ng/ml |
|
200 - 500 bp |
10 ng/ml |
|
500 - 1000 bp |
20 ng/ml |
|
1000 - 2000 bp |
40 ng/ml |
|
> 2000 bp |
100 ng/ml |
Primers should be
provided at a concentration of
3.2 Pico mol/ul
Total quantity required
Template
5.0ul
Primer
5.0ul
- Estimated time for results
will be 4-5 working days and
results will be given via
e-mail or in the form of print
outs of electropherograms.
Soft copies of data on
customer’s CD can also be
provided if requested.
The
electopherograms can be
visualized using the following
programs:
- ABI Sequencing Analysis
(Windows)
-
BioEdit (Windows)
·
Chromas 1.45
(Windows)
Manual editing of the sequence:
We manually verify
computer-generated base calling
to ensure the highest sequencing
accuracy.
