Important Tips in
Preparing Your
Material for DNA Sequencing
-
Please use HPLC grade water
-
Please purify your template with any
commonly used procedure.
Centricon-100 columns
are
recommended for this purpose.
-
Please run your template on agarose
gel to ascertain its purity
-
Please quantitate purified DNA by
measuring the absorbance at
260 nm or by any other method
that gives reliable
measurement.
-
Please provide the template in
distilled water free of EDTA
and other salts, proteins,
RNA, or genomic DNA.
-
Use 1.5ml eppendroff tubes or 96-well
PCR plates for pooling your
samples.
-
Please transport samples in a
container containing ice.
-
Please also observe other information
given in the DNA sequencing
request form.
Important Note
For optimum results, purify the
PCR product before sending for
sequencing. In general, any
method that removes dNTPs,
primers and excessive salts etc.
should work. Centricon -100
columns are recommend for the
purification of the DNA
template.
Important
Tips in Preparing Your Material
for DNA Fragment Analysis
All information on request form must be completed at the time of
submission.
·
DNA samples must be clearly
labeled with information
corresponding to that given on
the request form.
·
High quality DNA samples are an
absolute necessity for good
results. For optimal quality it
is recommended that genomic DNA
be prepared using Qiagen kits or
cesium chloride.
·
We recommend pooling different
dyes after your PCR reaction.
·
Please Prepare and submit
samples in a 96 well plate or
PCR tubes with appropriate
concentrations and along with
filled genotyping request form.
·
Please cover plate or tube with
aluminum foil. If shipping
sample, please cover plate with
cape strip.
·
At least 25 base pair difference
is recommended for two fragments
pooled in a single well and
labeled with same dye.
·
Please provide at lease 5ml of
the PCR product.
·
DNA fragments must be conjugated
with one of the following dyes,
VIC, HEX, NED, TET, PET, FAM.
